A METHOD FOR THE DETERMINATION OF L-LACTIC ACID
نویسندگان
چکیده
منابع مشابه
Optimal conditions for the enzymatic determination of L-lactic acid.
A simple enzymatic method for the determination of lactic acid with muscle lactic dehydrogenase is described. L ( +) Lactate in amounts from 0.04 M to 0.4 , M can be directly measured by following the formation of reduced diphosphopyridine nucleotide at 340 m . The effect of varying the concentrations of reactants in the test system was studied, and the optimal concentrations for stoichiometric...
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A method was developed for the detection and isolation, within a population of lactic acid bacteria, of strains producing exclusively the l-(+)- isomer of lactic acid; the visual detection of colonies of these particular strains can be carried out directly on agar plates (50 to 70 colonies per plate). The method is based on an enzymatic stereospecific reaction involving d-(-)-lactate dehydrogen...
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As the result of some experience in the determination of lactic acid in blood, muscle, and solutions of sugar derivatives, we have introduced various modifications in the process by which it gains considerably in simplicity, speed, convenience, and reliability. The basis of the method is the oxidation of the lactic acid in boiling sulfuric acid solution to acetaldehyde by permanganate (l), aera...
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15 صفحه اولA Method for the Determination of Small Amounts of Lactic Acid
For quantities of lactic acid greater than 10 or 20 mg. the titration method of von Ftirth and Charnass (1) is available. For much smaller quantities, the only methods hitherto proposed have been calorimetric (2). With the disadvantages of calorimetric methods in mind, study was made of a titration method which gives satisfactory results with as little lactic acid as 0.2 mg. Chemical methods fo...
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ژورنال
عنوان ژورنال: The Journal of General and Applied Microbiology
سال: 1964
ISSN: 1349-8037,0022-1260
DOI: 10.2323/jgam.10.45